Anti-Human Complement component 4d (C4d) Monoclonal Antibody (clone 7H4) -0.5 mg (CAT#: CB-P337-AB) Datasheet

Product Type
Antibody
Description
The monoclonal antibody 7H4 (also known as clone M4d2) recognizes an epitope on the alpha 2 chain of human C4d. Complement factor C4 (MW 41 kDa), formerly known as Gg protein, is composed of α, β and γ chains. The classical pathway of complement and the mannose-binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4, resulting in the formation of C4a and C4b. The latter can be cleaved by factor I to produce C4c and C4d, all functional sites are lost in this step. The C4d activation fragment of C4 is an excellent marker for the activation of the classical complement pathway and MBL pathway. The thioester formed between the side chains of Cys1010 and Gln1013 in the C4d region of the α chain mediates covalent attachment to the target surface with activated form of C1s or MASP. In addition, C4d is highly homologous to C3d, which has more than 35% shared amino acid sequence. In many diseases, such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria, the high complement level of C4d in serum or plasma is significantly increased. C4d levels in the plasma of patients with various humoral autoimmune diseases may also increase, and it is known that complement activation occurs. The deposition of C4d in the capillaries around the renal tubules has been shown to be a sensitive marker of antibody-mediated (humoral) rejection in renal transplantation biopsy. The monoclonal antibody 7H4 recognizes C4, C4b and C4d. Antibody 7H4 cross-reacts with guinea pigs.
Disease
Autoimmunity, Nephrology
Size
100 µg
Species
Human
Cross reactivity
Guinea pig
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
7H4
Buffer
PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application
Frozen sections, Immuno assays, Western blot
Application Notes
For immunohistology and western blotting, diluents will be used, depending on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Target
Complement component 4d (C4d)
Alternative Names
Complement component 4d

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK