Description
Annexin V-FITC Apoptosis Detection Kit Plus is based on the following observations: Most cell types transfer membrane phosphatidylserine (PS) from the inner surface of the plasma membrane to the cell surface soon after initiating apoptosis. Once it reaches the cell surface, PS can be easily detected by staining with the fluorescent conjugate of Annexin V, which is a protein with a strong natural affinity for PS. The one-step dyeing process only takes 10 minutes. In addition, the assay can be performed directly on live cells without fixation.
Annexin V-FITC Apoptosis Detection Kit Plus includes Annexin V-FITC, SYTOX green dye and binding buffer. SYTOX green dye does not penetrate living cells and apoptotic cells, but makes necrotic cells have strong green fluorescence by binding cell nucleic acid. After staining the cell population with Annexin V-FITC and SYTOX Green dyes in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show a higher level of green fluorescence, and live cells show little or no Fluorescence. These populations can be easily distinguished by a flow cytometer with 488 nm argon ion laser excitation. Annexin V-FITC and SYTOX Green dyes both emit green fluorescence, and green fluorescence can be detected in the FL1 channel, thereby freeing up other channels for adding other probes in multicolor labeling experiments.
Features
•Simple one-step procedure; only takes ten minutes
•Fast and convenient
•The Annexin VFITC Plus kit contains sytox green dye (instead of PI), which has a higher green fluorescence level on necrotic cells than FITC.
Applications
Annexin V-FITC kit includes Annexin V-FITC for detecting apoptosis and also propidium iodide (PI) for detecting necrosis.
Size
25 Assays
100 Assays
400 Assays
Detection Method
Flow cytometry (Ex = 488 nm; Em = 530 nm).
Sample
Works with both adherent and suspension cells.
Protocol Length
Get results in 9 minutes.
Components
Annexin V-FITC
SYTOX Green Dye
Binding Buffer
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.
