mCherry Quantification Kit (CAT#: CB-P004-K) Datasheet

mCherry is a second-generation monomeric red fluorescent protein, originally isolated from sea anemones and corals, such as GFP or DsRed. mCherry is widely used as a fluorescent tracer in transfection and transgenic experiments due to its better performance in brightness, light stability and monomer structure. Since mCherry does not require other substrates or cofactors, mCherry can be easily detected under a fluorescence microscope. However, most of mCherry's imaging studies are only qualitative. Creative Biolabs' mCherry quantification kit provides a simple 96-well plate detection method that can quantify the expression level of mCherry in cells or tissues. Cells or tissues can be homogenized directly in mCherry Assay Buffer. The amount of mCherry is determined by comparing its fluorescence (Ex / Em = 587/610 nm) with the mCherry standard provided in the kit. Each kit provides enough reagents to perform up to 100 assays.

The procedure is simple and takes about 20 minutes
Fast and convenient

It detects a wide range of mCherry (0.01-1 ng/µl)