mCherry Quantification Kit (CAT#: CB-P004-K) Datasheet

Description
mCherry is a second-generation monomeric red fluorescent protein, originally isolated from sea anemones and corals, such as GFP or DsRed. mCherry is widely used as a fluorescent tracer in transfection and transgenic experiments due to its better performance in brightness, light stability and monomer structure. Since mCherry does not require other substrates or cofactors, mCherry can be easily detected under a fluorescence microscope. However, most of mCherry's imaging studies are only qualitative. Creative Biolabs' mCherry quantification kit provides a simple 96-well plate detection method that can quantify the expression level of mCherry in cells or tissues. Cells or tissues can be homogenized directly in mCherry Assay Buffer. The amount of mCherry is determined by comparing its fluorescence (Ex / Em = 587/610 nm) with the mCherry standard provided in the kit. Each kit provides enough reagents to perform up to 100 assays.
Features
The procedure is simple and takes about 20 minutes
Fast and convenient
Applications
It detects a wide range of mCherry (0.01-1 ng/µl)
Size
100 assays
Detection Method
Fluorescence (Ex/Em 587/610 nm)
Sample
Cells etc
Species Reactivity
Mammalian
Components
mCherry Assay Buffer
mCherry Standard
Shipping
Gel Pack
Storage
-20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.

Figure: (a) mCherry standard curve for the detection range between 0 and 100 ng/well. (B) mCherry standard curve, by using 1 ng/μlmCherry working solution to generate better sensitivity (0-10 ng/well) to generate a standard curve. RFU: The relative fluorescence unit after subtracting the fluorescence reading of the buffer (0 mCherry).

All products and services are for Research Use Only. Do Not use in humans.

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