Description
The Annexin V Apoptosis Detection Kit is based on the following observations: Soon after initiating apoptosis, cells transfer membrane phosphatidylserine (PS) from the inner surface of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V (a protein with high affinity for PS). The one-step dyeing process only takes 10 minutes. Detection can be analyzed by flow cytometry or fluorescence microscopy.
Features
•Simple one-step dyeing process, only 10 minutes
•Fast and convenient
•When performing annexin V-FITC and PI staining, the kit can distinguish between apoptosis and necrosis
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Size
25 Assays
100 Assays
400 Assays
Detection Method
Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy
Sample
Works with both adherent and suspension cells.
Protocol Length
Get results in 9 minutes.
Components
Annexin V-Cy5
1X Binding Buffer
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.
