Annexin V-Biotin Apoptosis Kit (CAT#: CB-P028-K) Datasheet

Description
The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that most cell types transfer membrane phospholipid phosphatidylserine (PS) from the inner surface of the plasma membrane to the cell surface shortly after initiating apoptosis. Once on the cell surface, PS can easily bind to biotin-conjugated annexin V, which has a strong natural affinity for PS. Annexin V-Biotin can be combined with any reagent that uses streptavidin or avidin dyes (for example (streptavidin) antiviral-luciferin, -peroxidase, -alkaline phosphatase (AP)) And -Beta-gal, etc.) combined with conventional dye staining for detection.
Features
•Simple one-step dyeing procedure in 30 minutes
•Fast and convenient
•Annexin V-Biotin can be combined with any reagents that use streptavidin or avidin dyes for detection using conventional dye staining.
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Size
25 Assays
100 Assays
400 Assays
Detection Method
Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy
Sample
Works with both adherent and suspension cells.
Species Reactivity
Mammalian
Protocol Length
Get results in 9 minutes.
Components
Annexin V-Biotin
1X Binding Buffer
Propidium Iodide (PI)
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.

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