GFP Quantitation Kit (CAT#: CB-P003-K) Datasheet

Description
Green fluorescent protein (GFP) is a spontaneous fluorescent protein. GFP has also been recombined and modified (called EGFP). GFP and EGFP have been widely used as valuable reporter molecules to visualize gene expression events in various cell types and organisms in vivo. Since GFP does not require other substrates or cofactors, GFP fluorescence can be easily detected under a fluorescence microscope. However, most GFP imaging studies are only qualitative. Because the quantitative analysis of GFP expression levels in cells or tissues is more informative and has a wide range of applications, Creative Biolabs has developed the GFP Quantit Kit, which can quantify GFP in the form of a 96-well plate. Cells or tissues can be homogenized directly in GFP analysis buffer. Determine the amount of GFP by comparing its fluorescence with that of a GFP standard. The kit can detect multiple GFP concentrations (0.01-10 µg/ml). A GFP quenching solution is also provided to determine the autofluorescence of cell or tissue extracts. Each kit provides enough reagents to perform up to 100 determinations, Ex / Em = 488/507 nm.
Features
The procedure is simple and takes about 20 minutes
Fast and convenient
Applications
It detects a wide range of GFP concentration (0.01-10 ug/ml)
Size
100 assays
Detection Method
Fluorescence (Ex/Em 488/507 nm)
Sample
Cells etc
Species Reactivity
Mammalian
Components
GFP Assay Buffer
GFP Standard (1 µg/µl)
GFP Quench Solution
Shipping
Gel Pack
Storage
-20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.

Figure: GFP Standard Curve: The Assay was performed following the kit procedure.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK