Sucrose Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P173-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' sucrose assay kit provides a convenient for specifically measuring the sucrose levels in various biological samples (such as serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted into glucose and fructose by invertase. Then the generated glucose is specifically oxidized to generate a product that reacts with the probe to generate color (λ = 570 nm) and Fluorometric (Ex / Em = 535/587 nm). The free glucose background can be corrected using appropriate controls. Other disaccharides (such as lactose and maltose) will not interfere with the measurement. This can detect sucrose concentrations of 0.0002-10 mM.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
0 µM - 10 µM
Sensitivity
> 0.2 µM
Sample
Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Enzyme Mix (lyophilized) 1 vial
Invertase (Lyophilized) 1 vial
OxiRed Probe in DMSO 1 x 200µl
Sucrose Assay Buffer 1 x 25ml
Sucrose Standard (Lyophilised) 1 x 100µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard Sucrose Assay. Different amounts of sucrose, lactose and maltose are assayed following the kit protocol. Data show that the Sucrose assay kit specifically detects sucrose, but not lactose or maltose.

All products and services are for Research Use Only. Do Not use in humans.

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