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CreaTag™ V-tag Glycopeptide Labeling and Enrichment Kit [For Glycan Labeling] (CAT#: CB-P297-K) Datasheet

Product Type
Kit
Description
The kit contains the reagents required to conjugate the V-tag dye to the amine moiety of the glycopeptide, and a dedicated solid phase extraction (SPE) column required to purify and enrich the labeled glycopeptide.
Features
Number of Samples: 24 separate analytical samples per kit.
Amount of Sample: From approximately 1 µg to 50 µg of glycoprotein per sample.
Suitable Samples: IgG single subclass glycoproteins. Other glycoproteins can be used.
Labeling Selectivity:One V-tag label for every IgG glycopeptide N-terminus
Applications
For the fluorophore labeling and enrichment of glycopeptides.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
Tag dye 0.53 mg in 150 µL DMSO
TFA 10% 5 mL
A cartridges 1 pack (24 cartridges)
pH 7.2 PBS Buffer tablet 1 tablet to make 1L
Handling Advice
Ensure that any glass, plastic-ware or solvents are free from unwanted peptidases, glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrates
Shipping
The product can be shipped at ambient temperature.
Storage
Store the V-tag dye at -20°C in the dark. Protect from sources of heat, light and moisture. Once used, extra dye solution can be frozen and re-used.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

Figure 1 Chromatogram of a V-tag labeled, trypsin digested IgG-1 antibody performed on a Thermo U3000 UHPLC using a BEH Glycan 2.1 x 150 mm column (Waters) with a 30 min gradient.

Figure 2 Negative ion mass spectrum of a V-tag labeled, trypsin digested IgG-1 antibody using a Bruker Autoflex MALDI instrument.

All products and services are for Research Use Only. Do Not use in humans.

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Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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