CreaTag™ V-tag Glycopeptide Labeling and Enrichment Kit [For Glycan Labeling] (CAT#: CB-P297-K) Datasheet

Glycoproteins and glycopeptides containing N-linked glycans

Tag dye 0.53 mg in 150 µL DMSO
TFA 10% 5 mL
A cartridges 1 pack (24 cartridges)
pH 7.2 PBS Buffer tablet 1 tablet to make 1L

Ensure that any glass, plastic-ware or solvents are free from unwanted peptidases, glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrates

The product can be shipped at ambient temperature.

Store the V-tag dye at -20°C in the dark. Protect from sources of heat, light and moisture. Once used, extra dye solution can be frozen and re-used.

Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.

For research use only. Not for human or drug use

Figure 1 Chromatogram of a V-tag labeled, trypsin digested IgG-1 antibody performed on a Thermo U3000 UHPLC using a BEH Glycan 2.1 x 150 mm column (Waters) with a 30 min gradient.

Figure 2 Negative ion mass spectrum of a V-tag labeled, trypsin digested IgG-1 antibody using a Bruker Autoflex MALDI instrument.