Product Type
Kit
Description
The kit contains the reagents required to conjugate the V-tag dye to the amine moiety of the glycopeptide, and a dedicated solid phase extraction (SPE) column required to purify and enrich the labeled glycopeptide.
Features
Number of Samples: 24 separate analytical samples per kit.
Amount of Sample: From approximately 1 µg to 50 µg of glycoprotein per sample.
Suitable Samples: IgG single subclass glycoproteins. Other glycoproteins can be used.
Labeling Selectivity:One V-tag label for every IgG glycopeptide N-terminus
Applications
For the fluorophore labeling and enrichment of glycopeptides.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
Tag dye 0.53 mg in 150 µL DMSO
TFA 10% 5 mL
A cartridges 1 pack (24 cartridges)
pH 7.2 PBS Buffer tablet 1 tablet to make 1L
Handling Advice
Ensure that any glass, plastic-ware or solvents are free from unwanted peptidases, glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrates
Shipping
The product can be shipped at ambient temperature.
Storage
Store the V-tag dye at -20°C in the dark. Protect from sources of heat, light and moisture. Once used, extra dye solution can be frozen and re-used.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use
