α(1-3,4)-fucosidase [For Glycan Release] (CAT#: CB-P271-K) Datasheet

Kit

α(1-3,4) Fucosidase cleaves the branched non-reducing terminal fucose and connects α(1-3) or α(1-4) to the N-acetyl of the terminal Gal-GlcNAc disaccharide structure Glucosamine. The presence of sialic acid (but not fucose) linked to galactose prevents cleavage.

Source: Isolated from Xanthamonas manihotis

EC: 3.2.1.51

Alternate Names: α-L-fucoside fucohydrolase, α-L-fucosidase, α-(1-3,4) fucosidase
Specific Activity:>2 U/mg
Activity: 0.5 U/mL

Molecular weight: 40,000 daltons

Formulation: The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl pH 7.5.

Specificity: Non-reducing terminal branched fucose when linked α(1-3) or α(1-4) to GlcNAc of a Gal-GlcNAc disaccharide structure. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.

Specific Activity Assay: One unit of Fucosidase activity is defined as the amount of enzyme required to cleave 1 µmole of fucose from Lewis X trisaccharide, 4-methylumbelliferyl glycoside in 1 minute at 37°C and pH 5.0. Lewis X trisaccharide is Gal β-(1-4)[Fuc α-(1-3)]GlcNAc.

Purity: Each lot of α(1-3,4) Fucosidase is tested for contaminating activities by incubating the enzyme for 24 hours at 37°C with the appropriate substrates; the detection limit of this assay is 5 µU/mL (IUB). A passing lot will have no detectable activity.

α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures.