α(1-3,4)-fucosidase [For Glycan Release] (CAT#: CB-P271-K) Datasheet

Product Type
Kit
Description
α(1-3,4) Fucosidase cleaves the branched non-reducing terminal fucose and connects α(1-3) or α(1-4) to the N-acetyl of the terminal Gal-GlcNAc disaccharide structure Glucosamine. The presence of sialic acid (but not fucose) linked to galactose prevents cleavage.
Features
Source: Isolated from Xanthamonas manihotis

EC: 3.2.1.51

Alternate Names: α-L-fucoside fucohydrolase, α-L-fucosidase, α-(1-3,4) fucosidase
Specific Activity:>2 U/mg
Activity: 0.5 U/mL

Molecular weight: 40,000 daltons

Formulation: The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl pH 7.5.

Specificity: Non-reducing terminal branched fucose when linked α(1-3) or α(1-4) to GlcNAc of a Gal-GlcNAc disaccharide structure. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.

Specific Activity Assay: One unit of Fucosidase activity is defined as the amount of enzyme required to cleave 1 µmole of fucose from Lewis X trisaccharide, 4-methylumbelliferyl glycoside in 1 minute at 37°C and pH 5.0. Lewis X trisaccharide is Gal β-(1-4)[Fuc α-(1-3)]GlcNAc.

Purity: Each lot of α(1-3,4) Fucosidase is tested for contaminating activities by incubating the enzyme for 24 hours at 37°C with the appropriate substrates; the detection limit of this assay is 5 µU/mL (IUB). A passing lot will have no detectable activity.
Applications
α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures.
Size
30 mU / 60 µL; Sufficient for up to 60 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
α(1-3,4)-Fucosidase in 20 mM Tris-HCl, 25 mM NaCl,(pH 7.5).
5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
Handling Advice
1. Add up to 1 nmole of oligosaccharide to a tube.
2. Add de-ionized water to a total of 15 µL.
3. Add 4 µL of 5x Reaction Buffer 5.0.
4. Add 1 µL of α(1-3,4)-Fucosidase.
5. Incubate for 1 hour at 37°C.
Storage
Storage: Store enzyme at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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