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Total Carbohydrate Assay Kit (Colorimetric) (CAT#: CB-P155-K) Datasheet

Product Type
Kit
Description
Total carbohydrate assay is a simple, sensitive and reliable for detecting almost all carbohydrates.
The carbohydrate assay protocol is based on phenol-sulfuric acid assay. In this assay, polysaccharides (mono, di, third, etc.) and their derivatives are hydrolyzed into monomers in the presence of sulfuric acid and converted into furfural or hydroxyfurfural. They react with the developer to form chromogens, which can be quantified by measuring the absorbance at λ = 490 nm.
The total carbohydrate assay kit can detect most forms of carbohydrates, including simple and complex carbohydrates, glycans, glycoproteins and glycolipids.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Sample
Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
Carbohydrate Assay Buffer 1 x 25ml
Developer 1 x 3ml
D-Glucose Standard 1 x 0.2ml
Requires
Microplate reader
Handling Advice
Total carbohydrate assay protocol summary:
- add samples and glucose standards to wells
- add concentrated sulfuric acid and incubate for 15 min at 90°C
- add developer and incubate for 5 min
- analyze with a microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 D-Glucose standard curve generated using the method described in the protocol bookelt.

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All products and services are for Research Use Only. Do Not use in humans.

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