Sialic Acid (NANA) Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P138-K) Datasheet

Product Type
Kit
Description
The sialic acid (NANA) analysis kit is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.


The sialic acid assay uses an enzyme coupling reaction, in which the oxidation of free sialic acid produces an intermediate that reacts stoichiometrically with the probe. The generation can be determined by absorbance (OD = 570 nm) or Fluorometric (Ex / Em = 535). /587) The detected product nm).


The kit can measure sialic acid in a linear range of 0.1 to 10 nmol, with a assay sensitivity of about 1 µM concentration.
Applications
Functional Studies
Principle
This sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid. This reacts with thiobarbituric acid to form a pink colored product. The colorimetric (549nm)/fluorimetric (γex = 555nm/γem = 585nm) product is directly proportional to sialic acid concentration in the sample.
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
0.1 µM - 10 µM
Sensitivity
1 µM
Sample
Cell culture supernatant, Urine, Cell culture media
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
Sialic Acid Assay Buffer WM 1 x 25ml
Sialic Acid Converting Enzyme (lyophilized) Purple 1 vial
Sialic Acid Development Mix (lyophilized) Green 1 vial
Sialic Acid Probe (in DMSO) Red 1 x 200µl
Sialic Acid Standard (10 umol) (lyophilized)
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Concentrations of free SA decreased (P < 0.0001), whereas bound and total SA increased (P < 0.0001) across lactation.


Figure 2 Concentrations of free SA decreased (P < 0.0001), whereas bound and total SA increased (P < 0.0001) across lactation.


Figure 3 Concentrations of free SA decreased (P < 0.0001), whereas bound and total SA increased (P < 0.0001) across lactation.

All products and services are for Research Use Only. Do Not use in humans.

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