Sialic Acid (NANA) Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P138-K) Datasheet

Kit

The sialic acid (NANA) analysis kit is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.


The sialic acid assay uses an enzyme coupling reaction, in which the oxidation of free sialic acid produces an intermediate that reacts stoichiometrically with the probe. The generation can be determined by absorbance (OD = 570 nm) or Fluorometric (Ex / Em = 535). /587) The detected product nm).


The kit can measure sialic acid in a linear range of 0.1 to 10 nmol, with a assay sensitivity of about 1 µM concentration.

Functional Studies

This sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid. This reacts with thiobarbituric acid to form a pink colored product. The colorimetric (549nm)/fluorimetric (γex = 555nm/γem = 585nm) product is directly proportional to sialic acid concentration in the sample.