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Senescence Detection Kit (Fluorometric) (CAT#: CB-P022-K) Datasheet

Senescence is considered to be the tumor suppressor mechanism and the root cause of Senescence. Senescence represents a state of stagnation in which cells remain viable, but are not stimulated to divide by serum or passage in culture. Senescent cells show increased cell size, β-galactosidase (SA-Beta-Gal) activity and senescence-related expression and changes in gene expression patterns. The senescence detection kit is designed to detect SA-Beta-Gal activity in cultured cells by FACS fluorescence. SA-Beta-Gal is only found in senescent cells, and is not found in pre-senescence, resting or immortal cells.
Simple, one step protocol
Detection of senescence cells
100 assays
Detection Method
FACS (FL1 channel)
Cell culture (adherent and non adherent)
Species Reactivity
Wash Buffer
Senescence Dye
Gel Pack
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

Figure: 3T3 cells were seeded in 24 wells with 5x105 cells per well, and treated in complete cell culture medium at 37°C/5% for 4 hours (in the case of 200nM daunorubicin and HCl, respectively) In the test and control reactions) 48 hours of treatment. Carbon dioxide The medium was removed and replaced with a medium containing senescence dye, and incubated at 37°C/5% CO 2 for 2 hours. After the incubation time, the cells were washed twice with washing buffer, digested with trypsin, washed once with washing buffer and analyzed by FACS.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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