Figure: 3T3 cells were seeded in 24 wells with 5x105 cells per well, and treated in complete cell culture medium at 37°C/5% for 4 hours (in the case of 200nM daunorubicin and HCl, respectively) In the test and control reactions) 48 hours of treatment. Carbon dioxide The medium was removed and replaced with a medium containing senescence dye, and incubated at 37°C/5% CO 2 for 2 hours. After the incubation time, the cells were washed twice with washing buffer, digested with trypsin, washed once with washing buffer and analyzed by FACS.
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