Senescence Detection Kit (Fluorometric) (CAT#: CB-P022-K) Datasheet

Description
Senescence is considered to be the tumor suppressor mechanism and the root cause of Senescence. Senescence represents a state of stagnation in which cells remain viable, but are not stimulated to divide by serum or passage in culture. Senescent cells show increased cell size, β-galactosidase (SA-Beta-Gal) activity and senescence-related expression and changes in gene expression patterns. The senescence detection kit is designed to detect SA-Beta-Gal activity in cultured cells by FACS fluorescence. SA-Beta-Gal is only found in senescent cells, and is not found in pre-senescence, resting or immortal cells.
Features
Simple, one step protocol
Applications
Detection of senescence cells
Size
100 assays
Detection Method
FACS (FL1 channel)
Sample
Cell culture (adherent and non adherent)
Species Reactivity
Mammalian
Components
Wash Buffer
Senescence Dye
Shipping
Gel Pack
Storage
-20°C
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

Figure: 3T3 cells were seeded in 24 wells with 5x105 cells per well, and treated in complete cell culture medium at 37°C/5% for 4 hours (in the case of 200nM daunorubicin and HCl, respectively) In the test and control reactions) 48 hours of treatment. Carbon dioxide The medium was removed and replaced with a medium containing senescence dye, and incubated at 37°C/5% CO 2 for 2 hours. After the incubation time, the cells were washed twice with washing buffer, digested with trypsin, washed once with washing buffer and analyzed by FACS.

All products and services are for Research Use Only. Do Not use in humans.

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