Figure: 3T3 cells were seeded in 24 wells with 5x105 cells per well, and treated in complete cell culture medium at 37°C/5% for 4 hours (in the case of 200nM daunorubicin and HCl, respectively) In the test and control reactions) 48 hours of treatment. Carbon dioxide The medium was removed and replaced with a medium containing senescence dye, and incubated at 37°C/5% CO 2 for 2 hours. After the incubation time, the cells were washed twice with washing buffer, digested with trypsin, washed once with washing buffer and analyzed by FACS.
Related Products
All products and services are for Research Use Only. Do Not use in humans.
Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.