Description
Nuclear factor-κB (NFkB) is a transcription factor that plays a central role in many physiological processes, such as inflammation, tumorigenesis and apoptosis. NFkB is activated by a variety of stimuli, including inflammatory cytokines, such as TNF-α.
Creative Biolabs' cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to total protein content. This simple and effective assay method eliminates the need for cell lysate preparation, and can be used to study the regulation of NFkB in short-term and long-term assays.
Features
Simple. Without cell lysis, cells can be cultured for several days.
Applications
Used for quantitative fluorescence immunoenzyme method to determine the phosphorylation status of NFkB in cultured cells.
Size
100 tests
Detection Method
FL360/450nm, 530/585nm
Sample
Cell, tissue etc
Protocol Length
5hrs
Components
10x Wash Buffer: 25 mL, Blocking Buffer: 25 mL, Protein Stain: 6 mL, HRP Substrate: 6 mL, pNF-kappaB-Ab1: 10 uL HRP-Ab2 10 uL
Shipping
Gel Pack
Storage
Store all reagents at -20°C
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.
