Annexin V-PE-Cy5 Apoptosis Detection Kit (CAT#: CB-P038-K) Datasheet

The Annexin V-PE-Cy5 cell apoptosis detection kit is based on the following observations: Most cell types transfer membrane phosphatidylserine (PS) from the inner surface of the plasma membrane to the cell surface soon after initiating apoptosis. Once on the cell surface, PS can be easily detected by staining with the fluorescent conjugate of Annexin V, which is a protein with a strong natural affinity for PS. The one-step dyeing process only takes 10 minutes. In addition, the assay can be performed directly on live cells without fixation. The results can be analyzed by flow cytometry or fluorescence microscope.

Simple one-step procedure; only takes ten minutes
Fast and convenient, no need to fix cells

Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.