Description
The Annexin V-PE-Cy5 cell apoptosis detection kit is based on the following observations: Most cell types transfer membrane phosphatidylserine (PS) from the inner surface of the plasma membrane to the cell surface soon after initiating apoptosis. Once on the cell surface, PS can be easily detected by staining with the fluorescent conjugate of Annexin V, which is a protein with a strong natural affinity for PS. The one-step dyeing process only takes 10 minutes. In addition, the assay can be performed directly on live cells without fixation. The results can be analyzed by flow cytometry or fluorescence microscope.
Features
Simple one-step procedure; only takes ten minutes
Fast and convenient, no need to fix cells
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Size
25 Assays
100 Assays
400 Assays
Detection Method
Flow cytometry (Ex = 488 nm; Em = 670 nm) and fluorescence microscopy
Sample
Works with both adherent and suspension cells.
Protocol Length
Get results in 9 minutes.
Components
Annexin V-PE-Cy5
1X Binding Buffer
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.
