MBL Complement Pathway ELISA Kit [Human] (CAT#: CB-P060-K) Datasheet

Kit

The complement system plays an essential role in chronic, autoimmune and infectious disease. There are three pathways of complement activation, namely the classical, the alternative and the recently discovered MBL pathway. Impaired complement activity causes humans to become susceptible to repetitive fulminant or severe infections and may contribute to development of autoimmune disease. Inappropriate activation of complement contributes to chronic inflammation and tissue injury.

This kit is an enzyme immunoassay for determining the MBL complement pathway in human serum. For research use only, not for diagnostic procedures.

The complement assay Kit combines principles of the hemolytic assay for complement activation with the use of labeled antibodies specific for neoantigen produced as a result of complement activation. The amount of neoantigen generated is proportional to the functional activity of complement pathways. In the Complement system MP kit the wells of the microtitre strips are coated with specific activators of the MBL pathway. Subject serum is diluted in diluent containing specific blocker to ensure that only the MBL pathway is activated. During the incubation of the diluted subject serum in the wells, complement is activated by the specific coating. The wells are then washed and C5b-9 is detected with a specific alkaline phosphatase labelled antibody to the neoantigen expressed during MAC formation. After a further washing step, detection of specific antibodies is obtained by incubation with alkaline phosphatase substrate solution. The amount of complement activation correlates with the colour intensity and is measured in terms of absorbance (optical density (OD)).