Description
Cell death or cytotoxicity is classically assessed by quantitatively evaluating plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein found in all eukaryotic and prokaryotic cells. Once the plasma membrane is damaged, it will be quickly released into the culture medium. The bioluminescence cytotoxicity assay kit is based on the measurement of AK. This step is carried out in a simple one-step method involving two chemical reactions. The first reaction is the conversion of ADP to ATP by adenylate kinase released by damaged cells. The second reaction uses luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or beta counter. The assay is very sensitive and can be performed fully automatically to achieve high throughput.
Features
•Simple one-step procedure; less than 30 minutes
•Fast and convenient
•The assay is highly sensitive and can be fully automated for high-throughput research.
Applications
This kit provides a convenient and non-radioactive alternative to perform cytotoxicity assays.
Size
500 assays
Detection Method
Luminescence
Sample
Cell culture (adherent and non adherent)
Components
AK Detection Reagent (Lyophilized)
AK Assay Buffer
Shipping
Gel Pack
Storage
-20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.
