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iCrea™ ADCC mVEGF (-) Target Cell Line, Human HEK293 Cells [Control] (CAT#: AD-P022-C) Datasheet

iCrea™ ADCC Target mTNFα (-) Assay Cell Line are depleted of mTNFα expression, to be used as an internal control for unspecific activation of ADCC that can be a confounding factor when performing ADCC assays. These control cells can also be used for adjusting background levels when analyzing serum samples, thereby effectively removing any issues with varying background between samples iLite ADCC Target mVEGF (-) Assay Cell Line are based on a human embryonic kidney cell line, HEK293 ( CRL-1573), and have been genetically engineered and optimized to deplete all expression of the surface protein mVEGF. The cells are for use as internal controls together with iCrea™ ADCC Effector (V) Assay Cell Line and iCrea™ ADCC Target mVEGF (+) Assay Cell Line when measuring the ADCC activity of anti-TNF-alpha antibodies.
Read-out of results: Quantitative
Detection system: Luminescence
Regulatory status: Research Use Only
The iCrea™ mVEGF (-) Target Assay Cell Line can be used together with iCrea™ ADCC Effector (V) and iCrea™ mVEGF (+) Target Assay Cell Line for the quantification ADCC activity of anti-mVEGF antibodies.
1 vial corresponding to one 96 well plate
Species Reactivity
Cell Background
HEK293 Cells
Works with both adherent and suspension cells.
Growth Medium
Gibco (cat no 12648-010)
Cell Purity
Cell Viability
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
Guaranteed mycoplasma-free
Dry ice
Upon receipt confirm that adequate dry-ice is present, and the cells are
frozen. Immediately transfer to -80°C storage. Cells should be stored at
-80°C (do not store at any other temperature) and are stable as
supplied until the expiry date shown. Cells should be used within 30 min
of thawing.
Avoid multiple freeze/thaw cycles
Research Use Only
Our recombinant cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
The expression of hVISTA has been verified by flow cytometry.
The induction of antibody-dependent cellular cytotoxicity (ADCC) has been verified using anti-hVISTA-hIgG1 antibody and Jurkat-NFAT Lucia™ CD16 reporter cell line.
The stability of the 20 channels after thawing has been verified.
Raji-hVISTA cells are guaranteed to be free of mycoplasma.

Figure 1 Example of bevacizumab calibration curve using the firefly luciferase substrate luciferase reagent.

Figure 2
A: Measurement of the specific Firefly (FL) signal. Cells were stimulated with increasing concentrations of Trastuzumab.
B: Measurement of the Renilla (RL) signal for normalization, from the identical assay as in A.

Figure 3 Dose response curve after normalization of the specific Firefly signal with the according Renilla signal.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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