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iCrea™ ADCC CD16a (158V) (Luc) Reporter Cell Line, Human Jurkat Cells (CAT#: AES-1023-HM5) Datasheet

Description
Creative Biolabs developed and successfully established human CD16a (158V) (Luc) reporter cell line. The cells have been engineered to express not only the NFAT response elements that drive the luciferase expression system, but also express full length human CD16a receptor mutated to a Valine (V) at amino acid 158 that can be used to assess the ADCC activity of antibodies. CD16a (158V) exhibits a higher affinity for IgG1 and IgG3 isotypes compared to CD16a-158F.
Features
High sensitivity; Strong response signal; High stability
Protein of Expression
CD16a (158V)
Synonyms
CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; CD16-II; FCGRIII; FCRIIIA; FcGRIIIA
Protein Background
CD16a gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other responses, including antibody dependent cellular mediated cytotoxicity and antibody dependent enhancement of virus infections.
Species
Human
Applications
To assess the ADCC activity of antibodies
Size
5×10⁶ cells
Contents
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium.
Species Reactivity
Human
Cell Background
Jurkat T Lymphocytes
Selection Marker
Puromycin (5 μg/mL) + Hygromycin (20 μg/mL)
Growth Medium
RPMI-1640 + 10% FBS
Freeze Medium
Serum-free cell cryopreservation medium
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen in liquid nitrogen.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
Mycoplasma negative

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All products and services are for Research Use Only. Do Not use in humans.

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Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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