iCrea™ ADCC mTNF-alpha (-) Target Cell Line, Human HEK293 Cells [Control]

Description

iCrea™ ADCC Target mTNFα (-) Assay Cell Line are depleted of mTNFα expression, to be used as an internal control for unspecific activation of ADCC that can be a confounding factor when performing ADCC assays. These control cells can also be used for adjusting background levels when analyzing serum samples, thereby effectively removing any issues with varying background between samples iLite ADCC Target mTNF-alpha (-) Assay Cell Line are based on a human embryonic kidney cell line, HEK293 ( CRL-1573), and have been genetically engineered and optimized to deplete all expression of the surface protein mTNF-alpha. The cells are for use as internal controls together with iCrea™ ADCC Effector (V) Assay Cell Line and iCrea™ ADCC Target mTNF-alpha (+) Assay Cell Line when measuring the ADCC activity of anti-TNF-alpha antibodies.

Features

Read-out of results: Quantitative
Detection system: Luminescence
Regulatory status: Research Use Only

Applications

The iCrea™ mTNF-alpha (-) Target Assay Cell Line can be used together with iCrea™ ADCC Effector (V) and iCrea™ mTNF-alpha (+) Target Assay Cell Line for the quantification ADCC activity of anti-mTNF-alpha antibodies.

Size

1 vial corresponding to one 96 well plate

Species Reactivity

Human

Cell Background

HEK293 Cells

Sample

Works with both adherent and suspension cells.

Growth Medium

Gibco (cat no 12648-010)

Cell Purity

>95%

Cell Viability

>90%

Sterility Testing

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity Testing

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

Virological Safety Testing

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

Genetic Stability Testing

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

Mycoplasma Testing

Guaranteed mycoplasma-free

Shipping

Dry ice

Storage

Upon receipt confirm that adequate dry-ice is present, and the cells are
frozen. Immediately transfer to -80°C storage. Cells should be stored at
-80°C (do not store at any other temperature) and are stable as
supplied until the expiry date shown. Cells should be used within 30 min
of thawing.

Warnings

Avoid multiple freeze/thaw cycles

Research Use Only

Our recombinant cell are for research use only, not for diagnostic or therapeutic use.

Quality Control

The expression of hVISTA has been verified by flow cytometry.
The induction of antibody-dependent cellular cytotoxicity (ADCC) has been verified using anti-hVISTA-hIgG1 antibody and Jurkat-NFAT Lucia™ CD16 reporter cell line.
The stability of the 20 channels after thawing has been verified.
Raji-hVISTA cells are guaranteed to be free of mycoplasma.

Figure 1 Example of Infliximab calibration curve using the firefly luciferase substrate luciferase reagent.

Figure 2
A: Measurement of the specific Firefly (FL) signal. Cells were stimulated with increasing concentrations of Trastuzumab.
B: Measurement of the Renilla (RL) signal for normalization, from the identical assay as in A.

Figure 3 Dose response curve after normalization of the specific Firefly signal with the according Renilla signal.

iCrea™ ADCC mTNF-alpha (-) Target Cell Line, Human HEK293 Cells [Control] (CAT#: AD-P020-C) Datasheet

Overview

Specification

Images

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