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iCrea™ ADCP CD32a (131R) (Luc) Reporter Cell Line, Human Jurkat Cells (CAT#: AES-1023-HM3) Datasheet

Creative Biolabs developed and successfully established human CD32a (131R) reporter cell line. The cells have been engineered to express not only the NFAT response elements that drive the luciferase expression system, but also express the receptor full length human CD32a mutated to an Arginine (R) at amino acid 131 that can be used to assess the ADCP activity of antibodies. CD32a (131R) exhibits a lower affinity for IgG2 isotypes compared to CD32a-131H.
High sensitivity; Strong response signal; High stability
Protein of Expression
CD32a (131R)
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1; FcgammaRIIa
Protein Background
CD32a is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
To assess the ADCP activity of antibodies
5×10⁶ cells
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium.
Species Reactivity
Cell Background
Jurkat T Lymphocytes
Selection Marker
Puromycin (5 μg/mL) + Hygromycin (20 μg/mL)
Growth Medium
RPMI-1640 + 10% FBS
Freeze Medium
Serum-free cell cryopreservation medium
Cell Purity
Cell Viability
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Dry Ice
Frozen in liquid nitrogen.
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
Mycoplasma negative

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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