ADCP FcγRIIa (H variant) /NFAT Reporter Effector Cell Line, Jurkat T Lymphocytes (CAT#: AD-P011-C) Datasheet

Antibody-dependent cell-mediated phagocytosis (ADCP) is an important mechanism of action (MOA) of therapeutic antibodies. In vivo, ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells through FcyRIIa, FcyRI and FcyRIIIa. Although all three receptors can participate in ADCP, it is believed that FcγRIIa is the main Fcγ receptor involved in this process.

The FcγRIIa-HADCP Reporter bioassay is a biologically based MOA-based assay that can be used to measure the potency and stability of antibodies and other biological agents that specifically bind and activate FcγRIIa. The assay kit consists of Jurkat cells stably expressing human FcγRIIa-H (high affinity H131 variant) and NFAT-induced luciferase. Recombinant Jurkat T cells expressing the firefly luciferase gene under the control of the NFAT response element have the constitutive expression of human FcγRIIa, histidine variant.

Measure Potency and Stability of Biobetter Antibodies that Bind and Activate ADCP through FcγRIIa-H