iCrea™ ADCC CD20 (-) Target Cell Line, Human Raji Cells [Control] (CAT#: AD-P014-C) Datasheet

Description
iCrea™ ADCC Target CD20 (-) Assay Cell Line are depleted of CD20 expression, to be used as a control for unspecific activation of ADCC that can be a confounding factor when performing ADCC assays. iLite ADCC Target CD20 (-) Assay Cell Line are based on a human B lymphocyte cell line, Raji (ATCC# CCL-86), and have been genetically engineered and optimized to be depleted of CD20 expression. The cells are to be use as negative controls together with iCrea™ ADCC Effector (V) Assay Cell Line and iCrea™ ADCC Target CD20 (+) Assay Cell Line for measuring the ADCC activity of anti-CD20 antibodies.
Features
Read-out of results: Quantitative
Detection system: Luminescence
Regulatory status: Research Use Only
Applications
The iCrea™ CD20 (-) Target Assay Cell Line can be used together with iCrea™ ADCC Effector (V) and iCrea™ CD20 (+) Target Assay Cell Line for the quantification ADCC activity of anti-CD20 antibodies.
Size
1 vial corresponding to one 96 well plate
Species Reactivity
Human
Cell Background
Raji cell line
Sample
Works with both adherent and suspension cells.
Growth Medium
Gibco (cat no 12648-010)
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
Guaranteed mycoplasma-free
Shipping
Dry ice
Storage
Upon receipt confirm that adequate dry-ice is present, and the cells are
frozen. Immediately transfer to -80°C storage. Cells should be stored at
-80°C (do not store at any other temperature) and are stable as
supplied until the expiry date shown. Cells should be used within 30 min
of thawing.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
Our recombinant cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
The expression of hVISTA has been verified by flow cytometry.
The induction of antibody-dependent cellular cytotoxicity (ADCC) has been verified using anti-hVISTA-hIgG1 antibody and Jurkat-NFAT Lucia™ CD16 reporter cell line.
The stability of the 20 channels after thawing has been verified.
Raji-hVISTA cells are guaranteed to be free of mycoplasma.

Figure 1 Example of Trastuzumab calibration curve using the firefly luciferase substrate luciferase reagent.

Figure 2
A: Measurement of the specific Firefly (FL) signal. Cells were stimulated with increasing concentrations of Trastuzumab.
B: Measurement of the Renilla (RL) signal for normalization, from the identical assay as in A.

Figure 3 Dose response curve after normalization of the specific Firefly signal with the according Renilla signal.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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