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D-Mannitol Assay Kit (Colorimetric) (CAT#: CB-P161-K) Datasheet

Product Type
Kit
Description
In Creative Biolabs' D-mannitol assay kit (colorimetric), in the presence of NAD, mannitol dehydrogenase converts D-mannitol into D-fructose to form NADH, thereby reducing the colorless probe to A chromogen with strong absorption at 450 nm. The kit can also be used to measure another sugar alcohol, L-arabitol, because L-arabitol serves as a substrate for mannitol dehydrogenase. The D-mannitol assay kit is fast (~20 minutes), sensitive and easy to use. This assay kit can detect mannitol levels below 1 nmol/reaction (<10μM) and can be used for a variety of sample types.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Detection Limit
0 nmol/well - 20 nmol/well
Sensitivity
< 10 µM
Sample
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
Mannitol Assay Buffer 1 x 27ml
Mannitol Enzyme Mix 1 x 30µl
Mannitol Standard 1 vial
Mannitol Substrate Mix 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Mannitol measured in various samples showing quantity (nmol) per mg of extracted protein.


Figure 2 Measurement of Mannitol in the extracts of Longyan & Lyche.


Figure 3 Mannitol Standard Curve

All products and services are for Research Use Only. Do Not use in humans.

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