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D-Mannitol Assay Kit (Colorimetric) (CAT#: CB-P161-K) Datasheet

Product Type
Kit
Description
In Creative Biolabs' D-mannitol assay kit (colorimetric), in the presence of NAD, mannitol dehydrogenase converts D-mannitol into D-fructose to form NADH, thereby reducing the colorless probe to A chromogen with strong absorption at 450 nm. The kit can also be used to measure another sugar alcohol, L-arabitol, because L-arabitol serves as a substrate for mannitol dehydrogenase. The D-mannitol assay kit is fast (~20 minutes), sensitive and easy to use. This assay kit can detect mannitol levels below 1 nmol/reaction (<10μM) and can be used for a variety of sample types.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Detection Limit
0 nmol/well - 20 nmol/well
Sensitivity
< 10 µM
Sample
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
Mannitol Assay Buffer 1 x 27ml
Mannitol Enzyme Mix 1 x 30µl
Mannitol Standard 1 vial
Mannitol Substrate Mix 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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