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A3 Glycan (A3G3S3), Procainamide labelled [Labeled Glycan Standards] (CAT#: CB-P410-K) Datasheet

Product Type
Glycan Standards
Tri-sialylated, tri-antennary complex N-glycan (oligosaccharide). The product provided is a mixture of isomers with different types of sialic acid bonds (Neu5Acα2-3 or Neu5Acα2-6) and Gal residue bonds in one arm (Galβ1-4 or Galβ1-3). This is described in more detail below.
Sources: A3 glyans are found on several mammalian glycoproteins including bovine serum fetuin (bSF). This product is typically purified from the oligosaccharide pool released from bSF by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution containing approximately 50 mmol dm-3 of ammonium acetate (added to minimize acid-catalysed desialylation).
Molecular Weight: 2881
Purity:> 90% pure as assessed by a combination of 1 H-NMR and HPLC.
Glycoproteins and glycopeptides containing N-linked glycans
Handling Advice
Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates.
Refridgerate (-20°C) both before and after dissolution. This product is stable for at least 5 years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

Figure 1 Structure

Figure 2 HPAE-PAD HPLC Profile of A3 Glycan

All products and services are for Research Use Only. Do Not use in humans.


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