Glycogen Assay Kit II (Colorimetric) (CAT#: CB-P146-K) Datasheet

Product Type
Kit
Description
The Glycogen Assay Kit II (colorimetric) provides a simple, fast and reliable to measure the glycogen content in biological samples.
This glycogen assay is suitable for measuring the glycogen content in samples containing reducing substances, which may interfere with oxidase-based assays (such as our most popular glycogen assay kit.
In this glycogen assay protocol, glycogen is hydrolyzed to glucose, and then oxidized to form an intermediate, which reduces the colorless probe to a colored product with strong absorption at 450 nm. This high-throughput suitable assay kit can detect 4-40 µg/ml glycogen in samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Detection Limit
4 µg/ml - 40 µg/ml
Sensitivity
> 4 µg/ml
Sample
Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Development Enzyme Mix (lyophilized) 1 vial
Glycogen Development Buffer 1 x 25ml
Glycogen Hydrolysis Buffer 1 x 25ml
Glycogen Standard (2.0 mg/ml) 1 x 100µl
Hydrolysis Enzyme Mix (lyophilized) 1 vial
Probe 1 vial
Requires
Microplate reader
Handling Advice
Glycogen assay protocol summary:
- add samples and standards to wells
- add hydrolysis enzyme and incubate for 30 min at room temp
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glycogen measured in cell lysates showing quantity (µg) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL (HeLa) and 6.6e6 cells/mL (HCT116) were used. Samples were diluted 2-6 fold.


Figure 2 Glycogen measured in tissue lysates showing quantity (µg) per mg of extracted protein.
Protein concentration for samples varied from 25 mg/mL to 50 mg/mL. Samples were diluted 2-54 fold.


Figure 3 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 4 Glycogen level was highest in Alstonville larvae (n = 10 biological rep/mitotype).

All products and services are for Research Use Only. Do Not use in humans.

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