Sialidase Au α(2-3,6,8,9) [For Glycan Release] (CAT#: CB-P257-K) Datasheet

Kit

Α(2-3,6,8,9) sialidase cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. In addition, the enzyme will also cleave branched sialic acid (and internal The residues are connected).
α(2-3,6,8,9) sialidase Au can cleave all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rate of different bonds is: α(2-6)>α(2-3)>α(2-8), α(2-9).

In addition, the enzyme will cleave branched sialic acid (linked to internal residues). This characteristic makes it unique in sialidase. Cleavage of branch residues may require high concentrations of enzymes and extended incubation times. To cut only the non-reducing end α(2-3) unbranched sialic acid residues, use sialidase SP (catalog number CB-P258-K).
α(2-3,6,8,9) sialidase Au was isolated from the clone of Arthrobacter urea. The enzyme has been extensively characterized using oligosaccharide standards.

Source: Recombinant from Arthrobacter ureafaciens in E. Coli.

EC: 3.2.1.18

Alternate Names: Neuraminidase, NANase, N-acetylneuraminate glycohydrolase, Exo-α-sialidase
Specific Activity:>135 U/mg
Activity:>5 U/mL

Molecular weight: 70,000 daltons

pH range: 4.5-7, optimum 6.0
The recommended buffer concentrate provides the optimal pH for enzyme activity with the standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.

Specificity: Cleaves all non-reducing terminal branched and unbranched sialic acids

Specific Activity: Defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA [2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid].

Purity: Each lot of α(2-3,6,8,9) Sialidase Au is tested for contaminating protease as follows; 10 µg of denatured BSA is incubated for 24 hours with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.