Hexokinase Inhibitor Assay Kit (Colorimetric) (CAT#: CB-P189-K) Datasheet

Product Type
Kit
Description
The Hexokinase Inhibitor Screening Kit (colorimetric) provides a fast, simple and adaptable test that can perform high-throughput screening of hexokinase inhibitors within 30 minutes. Hexokinase converts glucose into glucose 6-phosphate. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, thereby reducing the colorless probe to a colored product with strong absorbance at OD = 450 nm. In the presence of hexokinase inhibitors, the reaction is prevented/eliminated, resulting in a decrease or complete loss of absorbance.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
HK Assay Buffer 1 x 25ml
Hexokinase (0.5 U) 1 vial
HK Coenzyme (25 mg) 1 vial
HK Converter (10 U) 1 vial
HK Developer (30 mg) 1 vial
HK Inhibitor Control (40 µmole) 1 vial
HK Substrate 1 x 1ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Typical inhibition curve of Hexokinase activity by Hexokinase Inhibitor (Bromopyruvic Acid).

All products and services are for Research Use Only. Do Not use in humans.

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