FcγRIIa-H ADCP Assay Kit (CAT#: CB-P055-K) Datasheet

Antibody-dependent cell-mediated phagocytosis (ADCP) is an important mechanism of action (MOA) of therapeutic antibodies. In vivo, ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells through FcyRIIa, FcyRI and FcyRIIIa. Although all three receptors can participate in ADCP, it is believed that FcγRIIa is the main Fcγ receptor involved in this process.

The FcγRIIa-HADCP Reporter bioassay is a biologically based MOA-based assay that can be used to measure the potency and stability of antibodies and other biological agents that specifically bind and activate FcγRIIa. The assay consists of Jurkat cells stably expressing human FcγRIIa-H (high affinity H131 variant) and NFAT-induced luciferase.

Compared with traditional methods, the cell format used after thawing can save a lot of time and labor while reducing variability.
The kit includes all necessary standard format reagents.
Easily adapt to high-throughput workflows.