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FcγRIIa-H ADCP Assay Kit (CAT#: CB-P055-K) Datasheet

Description
Antibody-dependent cell-mediated phagocytosis (ADCP) is an important mechanism of action (MOA) of therapeutic antibodies. In vivo, ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells through FcyRIIa, FcyRI and FcyRIIIa. Although all three receptors can participate in ADCP, it is believed that FcγRIIa is the main Fcγ receptor involved in this process.

The FcγRIIa-HADCP Reporter bioassay is a biologically based MOA-based assay that can be used to measure the potency and stability of antibodies and other biological agents that specifically bind and activate FcγRIIa. The assay consists of Jurkat cells stably expressing human FcγRIIa-H (high affinity H131 variant) and NFAT-induced luciferase.
Features
Compared with traditional methods, the cell format used after thawing can save a lot of time and labor while reducing variability.
The kit includes all necessary standard format reagents.
Easily adapt to high-throughput workflows.
Sample
Cells etc
Species Reactivity
Mammals, Other species
Components
Complete kit
Including FcγRIIa-H effector cells, target cells (Raji), control antibodies, cell culture media and assay reagents.
Recommended as a starter kit.

Core kit
Including FcγRIIa-H effector cells, cell culture media and assay reagents.
It is recommended to be used routinely with customer-defined antibodies and target cells.
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from date of purchase, if stored properly.
Research Use
For Research Use Only! Not For Use in Humans.

Assay Principle

The Fc effector reporter bioassay uses an alternative reading based on the traditional primary cell-based assay: FcγR and NFAT-mediated activation of luciferase activity in effector cells. The primary cells were replaced by Jurkat cell lines stably expressing human FcγRIIaH-131 and NFAT-induced luciferase. After binding to the Fc region of the relevant antibody bound to the target cell, ADCP bioassay effector cells expressing FcγRIIa-H131 have transduced intracellular signals, which can easily quantify the NFAT-RE-mediated luciferase activity.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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