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Complement TCC ELISA Kit [Human] (CAT#: CB-P065-K) Datasheet

Product Type
Kit
Description
In all cases where complement function needs to be determined, TCC levels can be very useful as a supplement to the evaluation of the function of the three complement pathways. It reflects the historical in vivo activity of complement in a given sample. The complement system plays an important role in chronic, autoimmune and infectious diseases. There are three pathways for complement activation, namely the classical pathway, the alternative pathway and the lectin pathway. The soluble terminal complement complex is the product of the terminal pathway and can be the result of all three complement activation pathways. Since TCC can reflect the activation response to the end of the final terminal pathway, regardless of the initial pathway involved, it is a particularly good candidate for general evaluation of complement activation. As we all know, under various pathological conditions, the complement system plays a key role in the development and amplification of the inflammatory process at the tissue level. Elevated TCC levels can be detected in, for example, hemolytic uremic syndrome (HUS), systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). The complement system can also be activated by artificial surfaces, such as during hemodialysis or extracorporeal circulation, which leads to increased TCC levels. TCC is also very suitable for studying the complement activation effect of biomaterials in medical devices. TCC can be measured in an assay using neoepitope-specific monoclonal antibodies like other complement activation products. New epitopes are hidden in natural complement components and are exposed after complement activation.
Applications
Complement TCC is an enzyme immunoassay for the semi-quantitative determination of soluble terminal complement complex (sTCC, also known as TCC or sC5b-9) in human EDTA plasma. This product is for professional use only. The results should not be used for clinical diagnosis or patient management. For research use only.
Principle
0
Size
1×96 wells
Detection Method
Enzyme immunoassay (ELISA) technique
Detection Limit
10 ng/mL - 400 ng/mL
Sensitivity
3 ng/ml
Sample
EDTA plasma
Specimen Collection
Blood samples were collected using sterile venipuncture techniques, and serum was obtained using standard procedures. At least 5 ml of whole blood is recommended. Let the blood clot in the serum test tube for 60-65 minutes at room temperature (20-25°C). Centrifuge the blood sample and transfer the cell-free serum to a clean test tube. The serum must be handled correctly to prevent complement activation in vitro. Serum should be frozen in a sealed tube at -70°C or lower for long-term storage or transport on dry ice. The sample should not be frozen and thawed more than once. Do not use jaundice, lipemia and hemolytic serum. You cannot use heat-inactivated serum. Plasma cannot be used.
Sample Volume
30 µl
Species Reactivity
Human
Protocol Length
2 hours
Storage
2 - 8 °C
Research Use
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

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All products and services are for Research Use Only. Do Not use in humans.

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