B3 Galactosyl Transferase [Enzymes] (CAT#: CB-X004-K) Datasheet

4mU

We recommend the use of Low Protein Binding Polypropylene Tubes (0.5-1.5 mL)
1. Add to a polypropylene tube:
- 5 µl HEPES Buffer at 500 mM, pH 7.5
- 0.5 µL 0.5 M MnCl2
- Acceptor molecule to a final concentration between 0.5 mM and 5 mM.
- UDP-galactose to a final concentration at least 1.5 times higher than acceptor.
- 10 µL of B3GalT (add enzyme always in the last place)
2. Mix by gently tapping the tube
3. Incubate at 37°C for 1 hour.
4. Analyze results by HPLC, UHPLC or MALDI-TOF.

Store at -20 °C>1 year. Avoid repeated freeze-thaw cycles, which decrease protein stability.

>1 yr at -20 °C.

For research use only. Not for human or drug use

Figure 1 Structure

Figure 2 Galactosyl Transferase Reaction