Mouse FcγRIV ADCC Assay Kit (CAT#: CB-P054-K) Datasheet

Description
Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) of antibodies that target virus-infected or diseased (eg, tumor) cells for destruction by components of the cell-mediated immune system. Mouse FcγRIV (mFcγRIV) is the main receptor involved in mouse ADCC and is closely related to human FcγRIIIa, which is the main Fc receptor involved in human ADCC.

Creative Biolabs' mFcγRIVADCC Reporter bioassay is a biologically based MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate FcγRIV. It is known that mouse IgG2a and to a lesser extent IgG2b mediate ADCC by activating mFcγRIV. In contrast, mouse IgG1 does not bind to mFcγIV. This bioassay overcomes the limitations of labor-intensive and highly variable primary cell assays. The workflow of this bioassay is simple and powerful, compatible with 96-well and 384-well plates, and unlike traditional primary cell-based assays, it provides a quantitative ADCC measurement method with low variability and high accuracy.
Applications
Demonstrate antibody ADCC activity.

Confirm mFcγRIV-mediated MOA.

Measure antibody potency and stability.

Screen and rank-order antibodies.
Sample
Cells etc
Species Reactivity
Mouse
Components
Complete kit
Including mFcγRIV effector cells, target cells (Raji), control antibodies, cell culture media and assay reagents.
Recommended as a starter kit.

Core suite
Including mFcγRIV effector cells, cell culture medium and assay reagents.
It is recommended to be used routinely with customer-defined antibodies and target cells.
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from date of purchase, if stored properly.
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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