Mouse FcγRIV ADCC Assay Kit (CAT#: CB-P054-K) Datasheet

Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) of antibodies that target virus-infected or diseased (eg, tumor) cells for destruction by components of the cell-mediated immune system. Mouse FcγRIV (mFcγRIV) is the main receptor involved in mouse ADCC and is closely related to human FcγRIIIa, which is the main Fc receptor involved in human ADCC.

Creative Biolabs' mFcγRIVADCC Reporter bioassay is a biologically based MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate FcγRIV. It is known that mouse IgG2a and to a lesser extent IgG2b mediate ADCC by activating mFcγRIV. In contrast, mouse IgG1 does not bind to mFcγIV. This bioassay overcomes the limitations of labor-intensive and highly variable primary cell assays. The workflow of this bioassay is simple and powerful, compatible with 96-well and 384-well plates, and unlike traditional primary cell-based assays, it provides a quantitative ADCC measurement method with low variability and high accuracy.

Demonstrate antibody ADCC activity.

Confirm mFcγRIV-mediated MOA.

Measure antibody potency and stability.

Screen and rank-order antibodies.