CytoTox™ Complement System Alternative Pathway Assay Kit (CAT#: CB-P208-K) Datasheet

96 wells

Specimen collection Blood samples are to be collected using aseptic venipuncture technique and serum obtained using standard procedures. A minimum of 5 mL of whole blood is recommended. Allow blood to clot in serum tubes, for 60-65 minutes at room temperature (20-25° C). Centrifuge blood samples and transfer cellfree serum to a clean tube. Sera must be handled properly to prevent in vitro complement activation. Sera should be frozen at -70° C or lower in tightly sealed tubes for extended storage or for transport on dry ice. Samples should not be frozen and thawed more than once. Avoid using sera which are icteric, lipemic and hemolyzed. Heat-inactivated sera cannot be used. Plasma can not be used.

Figure 1 AP assay.

Table 1.

Table 2 Sera with known complement deficiencies were tested in the assay and the following results were obtained. All deficient sera were detected in the assay and gave values below 5 %.

Table 3. Inter-assay precision was determined by testing three samples in duplicate. Results were obtained for six different runs.

Table 4. Intra-assay precision was determined by testing one sample in 40 wells.