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CytoTox™ Complement System Alternative Pathway Assay Kit (CAT#: CB-P208-K) Datasheet

Product Type
Kit
Description
The CytoTox™ Complement System Alternative Pathway kit is an immunoassay for the qualitative assay of functional alternative complement pathway and assay of complement deficiencies in human serum.

The CytoTox™ Complement assay combines the specific activation of the pathway with the use of labeled antibodies specific for a neoepitope of the terminal complement complex, C5b-9, produced as the result of complement activation. The amount of C5b-9 generated is proportional to the functional activity of the complement system. The convenient ELISA technology has several advantages compared to the traditional hemolytic assays.
Size
96 wells
Handling Advice
Specimen collection Blood samples are to be collected using aseptic venipuncture technique and serum obtained using standard procedures. A minimum of 5 mL of whole blood is recommended. Allow blood to clot in serum tubes, for 60-65 minutes at room temperature (20-25° C). Centrifuge blood samples and transfer cellfree serum to a clean tube. Sera must be handled properly to prevent in vitro complement activation. Sera should be frozen at -70° C or lower in tightly sealed tubes for extended storage or for transport on dry ice. Samples should not be frozen and thawed more than once. Avoid using sera which are icteric, lipemic and hemolyzed. Heat-inactivated sera cannot be used. Plasma can not be used.

Figure 1 AP assay.

Table 1.

Table 2 Sera with known complement deficiencies were tested in the assay and the following results were obtained. All deficient sera were detected in the assay and gave values below 5 %.

Table 3. Inter-assay precision was determined by testing three samples in duplicate. Results were obtained for six different runs.

Table 4. Intra-assay precision was determined by testing one sample in 40 wells.

All products and services are for Research Use Only. Do Not use in humans.

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