CreaTag™ V-TAG Glycan Release and labelling kit [For Glycan Labeling] (CAT#: CB-P295-K) Datasheet

Glycoproteins and glycopeptides containing N-linked glycans

rPNGase F release kit 1 kit (4 components)
PNGase F (Elizabethkingia miricola) supplied in 1 vial of 30 μL
50 mM NaCl 5 mM EDTA 20 mM Tris-HCl pH 7.5
10XGlyco Buffer 2 1 vial of 1.0 mL
500 mM sodium phosphate (pH 7.5 at 1X dilution)
10X Glycoprotein Denaturing Buffer 1 vial of 1.0 mL
5% SDS 400 mM DTT
10% NP-40 solution 1 vial of 1.0 mL
V-tag dye 1 ampoule (22 mg)
DMSO 1 ampoule (400 µL)
VSPE Cartridges 1 pack (30 cartridges)
ammonium formate Buffer, pH 4.4 1 bottle (3 mL)
ammonium acetate Buffer 1 bottle (38 mL)

Ensure that any glass, plastic ware or solvents used with this item are free of environmental carbohydrates and contaminating enzymes. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

The product should be shipped at 4°C.

Store at 4°C. Protect from sources of heat and light.

Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.

For research use only. Not for human or drug use

Figure 1 workflow.

Figure 2 Chromatogram of V-tag labelled glycans released from IgG after 10 minutes PNGase F digestion.

Figure 3 Chromatogram of V-tag labeled glycans released from bovine fetuin after 3 hours PNGase F digestion.