L-Lactate Assay Kit (Fluorometric), High Sensitivity (CAT#: CB-P168-K) Datasheet

Product Type
Kit
Description
The highly sensitive L-lactic acid assay kit is suitable for measuring very low L(+)-lactic acid levels in various samples.
In this assay, L(+)-lactic acid is specifically oxidized to form an intermediate, which reacts with a colorless probe to generate Fluorometric (Ex / Em = 535/587 nm), which is proportional to the amount of lactic acid.
This simple, fast, and high-throughput test kit is the most sensitive lactic acid test kit on the market. It can detect L(+)-lactic acid below 0.2 µM in various biological samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1000 nmol/well - 250000 nmol/well
Sensitivity
< 0.2 µM
Sample
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
L(+)-Lactate Standard (100mM) 1 x 100µl
Lactate Assay Buffer 1 x 25ml
Lactate Enzyme Mix (lyophilized) 1 vial
Lactate Substrate Mix 1 vial
PicoProbe 1 x 0.4ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve with background signal subtracted (duplicates; +/- SD).


Figure 2 Measurement of Lactate levels in rat liver (1.2 µg), kidney (0.7 µg), muscle (0.45 µg) and in human serum (0.5 µL from 1:10 diluted serum). Assays were performed following kit protocol.


Figure 3 L-Lactate measured in unfiltered human plasma (dilution range 1:30-1:90) and mouse serum (dilution range 1:2500-1:7500); duplicates +/- SD. The background control levels (no enzyme) were below level of detection.

All products and services are for Research Use Only. Do Not use in humans.

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