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CreaTag™ Quantitative Monosaccharide Release and 2-AA Labelling Kit [For Glycan Labeling] (CAT#: CB-P286-K) Datasheet

Product Type
Kit
Description
The kit contains reagents for releasing monosaccharides from glycoprotein biopharmaceuticals and standards. The released monosaccharide has a free reducing end and can be fluorescently labeled by reductive amination.
Features
Number of Samples: The kit contains reagents and materials for up to 96 glycoprotein samples analysed in
parallel or two sets of 48 samples.
Amount of Sample: Typically, around 50 µg of glycoprotein per sample dependent on levels of glycans
present which should be determined in a pilot study.
Suitable Samples: Biopharmaceutical glycoproteins.
Applications
For release of neutral and amino monosaccharides from glycoprotein therapeutics and pre-released glycans, and subsequent labeling with 2-aminobenzoic acid (2-AA).
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
Trifluoroacetic acid 2
Hydrochloric acid 2
Sodium acetate 2
Labeling solvent 2
2-aminobenzoic acid 2
Sodium cyanoborohydride 2
10 nmols each of glucosamine, galactosamine, galactose, glucose (dextrose) mannose and fucose 4
100 nmol of xylose 2
Handling Advice
Ensure that any glass, plastic ware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate. • Once individual vials of reagents are opened, their contents should be used immediately and excess then discarded according to local safety rules.
Storage
Store the whole kit at 4°C in the dark. Once monosaccharide standards are dissolved in solvent we recommend storing them at -20°C. Protect from sources of heat, light, and moisture. Use kit within 6 months of purchase.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

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All products and services are for Research Use Only. Do Not use in humans.

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