CreaZyme Acetyl Esterase (sialate-O-acetylesterase) Kit [For Glycan Release] (CAT#: CB-P264-K) Datasheet

Kit

Acetyl esterase (sialic acid-O-acetyl esterase) is a recombinant protein derived from standard reference strain of Tannerella forsythia and expressed in E. coli. The enzyme removes the acetyl groups attached via O-groups (mainly 9-, 8- and 7-). It can be used to monitor the diacetylation of sialic acid on products such as erythropoietin (EPO)

Source: Recombinant from Tannerella forsythia in E. Coli.

EC: 3.1.1.53

Number of Samples: Sufficient for up to 50 samples.

Amount of Sample: Up to 10 µg glycoprotein, up to 2.5 µg released glycans and up to 1 µg free sialic acid per digestion.

Suitable Samples: Acetyl esterase (sialate-O-acetylesterase) can act upon complex glycoprotein samples, such as erythropoietin (EPO), bovine submaxillary mucin and oral epithelial cell-bound glycans, and on N- and O-glycans released from a glycoprotein. Either fluorescently labelled or unlabelled glycans are suitable. It can also be used on released sialic acids.

Unit Definition: One unit (U) of acetyl esterase is defined as the amount of enzyme required to produce 300 µmole of 4-nitrophenol and acetate in 1 minute at 30°C in a buffer containing 50 mM Tris-HCl, 140 mM NaCl, pH 8.5, from 4-nitrophenyl acetate, a chromogenic esterase substrate.

Molecular Weight: 76.3 kDa.

To remove 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins. Used for characterisation of highly sialylated biotherapeutics such as EPO, FSH and blood clotting factors.