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6-Phosphofructokinase Activity Assay Kit (Colorimetric) (CAT#: CB-P154-K) Datasheet

Product Type
Kit
Description
The 6-phosphofructokinase activity assay kit is simple, reliable, and rapid, and can detect less than 1 mU of phosphofructokinase activity.
In the PFK activity assay protocol, PFK (phosphofructose kinase) converts fructose 6 phosphate and ATP into fructose diphosphate and ADP. In the presence of a substrate and enzyme mixture, ADP is converted to AMP and NADH, which reduces the colorless probe to a colored product with strong absorption at 450 nm.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Detection Limit
2 nmol/well - 10 nmol/well
Sensitivity
< 1 mU/well
Sample
Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
1h 30m
Components
ATP 1 vial
NADH Standard 1 vial
PFK Assay Buffer 1 x 27ml
PFK Developer 1 vial
PFK Enzyme Mix 1 vial
PFK Positive Control 1 vial
PFK Substrate 1 vial
Requires
Microplate reader
Handling Advice
PFK activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 PFK activity determined in bovine liver and HeLa cell lysate.

All products and services are for Research Use Only. Do Not use in humans.

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