α(1-3,6)-galactosidase [For Glycan Release] (CAT#: CB-P267-K) Datasheet

Kit

Alpha-galactosidase from Escherichia coli cleaves the non-reducing terminal galactose linked to α(1-3) and α(1-6) from complex carbohydrates and glycoproteins. For α(1-4) connection The galactose is inactive. It is particularly effective in removing α-linked galactose under conditions where the pH must be neutral or higher, such as for living cells.
In order to cleave β-galactosidase, we recommend purified β(1-4) galactosidase (CB-P265-K catalog), or recombinant from Streptococcus pneumoniae or β(1,3,4,6) galactosidase Recombinant of glycosidase (CB-P266-K catalog). From bovine testicles.

Source: Recombinant from E. coli in E. coli

EC: 3.2.1.22

Alternate Names: α-D-galactoside galactohydrolase, melibiase

Specific Activity:>30 U/mg
Activity: 80 U/mL

Molecular weight: ~80,000 daltons

Specificity: Cleaves α(1-3) and α(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

Specific Activity: One unit of α(1-3,6)-Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 25°C pH 6.5 from p-nitrophenyl-alpha-D-galactopyranoside.

α Galactosidase from E. coli cleaves α(1-3) and α(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.