CreaClean™ EC50 Glycan Clean-up Cartridges [For Glycan Clean Up] (CAT#: CB-P276-K) Datasheet

Kit

The cartridge contains a unique non-porous electronic interaction (EI) matrix. This is like a superhydrophobic resin that can even bind very hydrophilic polysaccharides. Most salts and detergents are either simply passed through the cartridge or lightly bound together, which can be washed off before eluting the glycans.

Binding Capacity: Each CreaClean™ EC50 cartridge can typically bind up to 50 µg of O- or N-linked
glycans.
Number of Samples: CreaClean™ EC50 cartridges are designed for single use only.
Suitable Samples: A wide range of glycans can be purified. These include N-linked and O-linked type
oligosaccharides, tri-saccharides and larger structures.
The cartridges are not suitable either for monosaccharides or disaccharides which are
generally bound too weakly for efficient purification or for large linear poly-sialylated
glycans which can be bound very tightly to the resin.
Glycan samples must be applied to the cartridges in solutions that are substantially
aqueous.
Structural Integrity: No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
Binding efficiency:> 95 % for most glycans

For purification of glycans from a variety of complex mixtures (including removal of salts and detergents).