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Alpha-Glucosidase Activity Assay Kit (Colorimetric) (CAT#: CB-P158-K) Datasheet

Product Type
Kit
Description
In Creative Biolabs' α-glucosidase activity assay kit (colorimetric), α-glucosidase hydrolyzes the substrate mixture and releases p-nitrophenol (OD = 410 nm) that can be measured by colorimetry. This is a simple, fast and high-throughput kit that can measure 0.1-10 mUα-glucosidase activity in various samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Sensitivity
0.1 mU/well - 10 mU/well
Sample
Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
Alpha Glucosidase Assay Buffer 1 x 25ml
Alpha Glucosidase Positive Control 1 vial
Alpha Glucosidase Substrate Mix 1 x 0.3ml
p-Nitrophenol Standard (100 mM) 1 x 100µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 2 Functional Studies- a-Glucosidase kinetic assay


Figure 3 Functional Studies
Time course of Alpha-Glucosidase Activity in mouse liver tissue lysate

All products and services are for Research Use Only. Do Not use in humans.

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