Complement Classical Pathway ELISA [Human] (CAT#: CB-P064-K) Datasheet

Product Type
Kit
Description
The complement system plays an important role in chronic, autoimmune and infectious diseases. There are three ways of complement activation, namely the classic way, the alternative way and the lectin way.

Impaired complement activity can lead to human susceptibility to repeated attacks or severe infections, and may lead to the development of autoimmune diseases. Improper complement activation can lead to chronic inflammation and tissue damage.

In vitro activation of the complement sequence leads to the consumption of complement components, which in turn leads to a decrease in their concentration. Therefore, the determination of complement protein or complement activity is used to indicate whether the complement system has been activated by immune and/or pathogenic mechanisms. When a complement activation disease or genetic defect is suspected, both functional and immunochemical complement measurements are used to evaluate the subject. The level of complement activity assessed by functional assays takes into account the rate of synthesis, degradation, and component consumption, and provides a measure of pathway integrity, as opposed to immunochemical methods that specifically measure the concentration of various complement components.
Applications
The classical complement pathway ELISA is an enzyme immunoassay for the qualitative and/or semi-quantitative determination of the functional classical complement pathway in human serum. The results should not be used for clinical diagnosis or patient management.
Principle
0
Size
1×96 wells
Detection Method
Enzyme immunoassay (ELISA) technique
Sensitivity
See Package Insert
Sample
Serum
Specimen Collection
Blood samples were collected using sterile venipuncture techniques, and serum was obtained using standard procedures. At least 5 ml of whole blood is recommended. Let the blood clot in the serum test tube for 60-65 minutes at room temperature (20-25°C). Centrifuge the blood sample and transfer the cell-free serum to a clean test tube. The serum must be handled correctly to prevent complement activation in vitro. Serum should be frozen in a sealed tube at -70°C or lower for long-term storage or transport on dry ice. The sample should not be frozen and thawed more than once. Do not use jaundice, lipemia and hemolytic serum. You cannot use heat-inactivated serum. Plasma cannot be used.
Sample Volume
10 µL
Species Reactivity
Human
Protocol Length
2 hours
Storage
2 - 8 °C
Research Use
For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product.

All products and services are for Research Use Only. Do Not use in humans.

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