ADP Assay Kit (Luminescent) (CAT#: CB-P009-K) Datasheet

Creative Biolabs' Lumi ADP Analysis Kit provides a fast method to measure ADP levels. The measurement involves two steps. In the first step, the working reagent lyses the cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the substrate D-luciferin to produce light. Light intensity is a direct measure of the ATP concentration in the cell. In the second step, ADP is converted to ATP through an enzymatic reaction. Then like the first step, this newly formed ATP reacts with D-luciferin. The measured second light intensity represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. This reagent is compatible with all media and all liquid handling systems, and can be used for high-throughput screening applications in 96-well and 384-well plates.

•Safety. Non-radioactive determination.
•Sensitive and accurate. It can be quantified as low as 0.02 ADP.
•Similar and convenient. Type determination of "mixed cultivation measures". No washing and reagent transfer steps are involved.
•Robust and suitable for HTS: The HTS liquid handling system can be easily automated and can process thousands of samples per day.

It is used for rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of the effect of drugs on ADP metabolism.