Description
Creative Biolabs' Lumi ADP Analysis Kit provides a fast method to measure ADP levels. The measurement involves two steps. In the first step, the working reagent lyses the cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the substrate D-luciferin to produce light. Light intensity is a direct measure of the ATP concentration in the cell. In the second step, ADP is converted to ATP through an enzymatic reaction. Then like the first step, this newly formed ATP reacts with D-luciferin. The measured second light intensity represents the total ADP and ATP concentration in the sample. This non-radioactive, homogeneous cell-based assay is performed in microplates. This reagent is compatible with all media and all liquid handling systems, and can be used for high-throughput screening applications in 96-well and 384-well plates.
Features
•Safety. Non-radioactive determination.
•Sensitive and accurate. It can be quantified as low as 0.02 ADP.
•Similar and convenient. Type determination of "mixed cultivation measures". No washing and reagent transfer steps are involved.
•Robust and suitable for HTS: The HTS liquid handling system can be easily automated and can process thousands of samples per day.
Applications
It is used for rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of the effect of drugs on ADP metabolism.
Size
100 tests
Detection Method
Luminescence
Detection Limit
0.02
Sample
Cells etc
Protocol Length
20 min
Components
Assay Buffer: 10 mL Substrate: 120 mL Cosubstrate: 120 mL ATP Enzyme: 120 mL ADP Enzyme: 120 mL Standard: 100 mL 3 mM ADP
Shipping
Gel Pack
Storage
Store all reagents at -20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.
