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Complement C4d Fixation Assay Capture Antibody (CAT#: CB-P197-K) Datasheet

Product Type
Antibody
Description
Complement C4d Capture antibody can be used to detect C4d. The antibody targets a neo-epitope on C4d (both A and B genetic variants) and does not bind to C4 or any other C4 fragments.
Size
20 µg (1.25 mg/mL)

Figure 1 Assay design.
It is recommended to set up the complement C4d Fixation Assay detection as a three-step sandwich detection, and use its designated function of capture and detection antibodies to ensure the best specificity for C4d new epitopes in EDTA plasma samples. The recommended analysis design is also used in Creative Biolabs's Complement C4d ELISA. The following describes a recommended assay protocol that allows automatic quantification of C4d using Svar Life Science's proprietary C4d antibody.


Fig.2 Recommended standard curve preparation. Note: the graph shows an example of a semi-quantitative standard curve and should not be used for actual subject sample interpretation.

Fig.3 Cross-reactivity with other complement factors in the Complement C4d Fixation Assay assay.

All products and services are for Research Use Only. Do Not use in humans.

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Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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