Complement C5 ELISA kit [Human]-2 x 96 det. (CAT#: CB-P072-K) Datasheet

Kit

Complement C5 (molecular weight is about 190kDa) is a key component of the complement system. This system plays an important role in both innate and adaptive immune responses, and can produce inflammatory and protective responses to pathogen attacks before adaptive responses occur. The complement system consists of a complex family of proteins and receptors, which are found in the circulatory system, tissues and other body fluids. Excessive/uncontrolled activation/regulation of the complement system involves many diseases, such as asthma, lupus erythematosus, glomerulonephritis, various forms of arthritis, autoimmune heart disease, multiple sclerosis, inflammatory bowel disease, Paroxysmal nocturnal hemoglobinuria, atypical hemolytic uremic syndrome and ischemia-reperfusion injury and transplant rejection. Three main pathways within the complement system have been described, namely the classical pathway, the alternative pathway and the lectin pathway, which are activated by different stimuli. The classical pathway is triggered by immune complexes. Through the lectin pathway of surface-bound lectins; and protect AP through all unprotected surfaces. All three pathways converge on complement C3. The cleavage of C3 induces the cleavage of C5, which in turn leads to the production of membrane attack complex (MAC). This protein complex is the ultimate common effector responsible for lysing susceptible microorganisms or destroying cells. Mutations in the C5 gene can cause a deficiency of complement component 5. This disease is characterized by repeated bacterial infections.

The Human C5 ELISA kit is used for the in vitro quantitative determination of C5 in plasma and serum samples.

The Human C5 ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle, with a working time of 3.5 hours.
The effective form of the plate with twelve disposable 8-well plates allows free choice of batch size for determination.
The samples and standards are incubated in microtiter wells coated with antibodies that recognize human C5.
The biotinylated tracer antibody will bind to the captured human C5.
 Streptavidin peroxidase conjugate will bind to the biotinylated tracer antibody.  Streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB).  The enzyme reaction can be stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. Draw a standard curve by plotting the absorbance (linear) relative to the corresponding concentration (logarithm) of the human C5 standard.
The concentration of human C5 in the sample that was performed at the same time as the standard can be determined from the standard curve.