Book a Meeting

Caspase 3/7 Assay Kit (Fluorometric) (CAT#: CB-P041-K) Datasheet

Description
Creative Biolabs' Caspase-3/7 Activity Assay Kit provides a convenient method to measure caspase-3 activity in biological samples. In the assay, a specific substrate (N-Ac-DEVD-AFC) is cleaved by active caspase-3 to form a highly fluorescent product. The fluorescence intensity (Ex / Em = 400 / 490nm) is directly proportional to the activity of caspase-3.
Features
•Safety. Non-radioactive determination.
•Convenient and high throughput. Homogeneous "mixed cultivation measures" type determination. The HTS liquid handling system can be easily automated and can process thousands of samples per day.
Applications
In order to quantitatively determine the apoptotic target caspase-3/7 activity, and use HTS to screen apoptosis inducers and inhibitors.
Size
100 tests
Detection Method
FL400/490nm
Sample
Cell and tissue lysate
Species Reactivity
All species
Protocol Length
60 min
Components
Assay Buffer: 12 mL Substrate: 240 mL DTT Solution: 240 mL
Requires
Pipetting devices, centrifuge tubes, black flat bottom 96-well plates and plate reader.
Shipping
Gel Pack
Storage
Store all reagents at -20°C.
Shelf Life
12 months after receipt.
Research Use
For Research Use Only! Not For Use in Humans.

Related Products

Anti-Mouse C3b/iC3b/C3c Monoclonal Antibody (clone 3/26) -100 µg (CAT#: CB-P241-AB)
Ficolin-3 ELISA kit [Human]-2 x 96 det. (CAT#: CB-P086-K)
Complement C4d Fixation Assay Detection Antibody (CAT#: CB-P198-K)
Anti-Mouse C5aR Monoclonal Antibody (clone 10/92) -0.5 mg (CAT#: CB-P395-AB)
Alternative Complement Pathway ELISA Kit [Human] (CAT#: CB-P058-K)
Anti-Mouse CR1/CR2 Monoclonal Antibody (clone 7E9) -100 µg (CAT#: CB-P238-AB)
Factor H, 402H/Y Variant ELISA Kit [Human], 2 x 96 det. (CAT#: CB-P108-K)
Anti-Human Properdin Monoclonal Antibody (clone 2.9) -0.5 mg (CAT#: CB-P325-AB)

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK

Germany

ISO 9001 Certified - Creative Biolabs Quality Management System.