Senescence Detection Kit (CAT#: CB-P021-K) Datasheet

Description
Aging is considered to be the tumor suppressor mechanism and the root cause of aging. Senescence represents a state of stagnation in which cells remain viable, but are not stimulated to divide by serum or passage in culture. Senescent cells show increased cell size, β-galactosidase (SA-Beta-Gal) activity and senescence-related expression and changes in gene expression patterns. The senescence detection kit is used to histochemically detect the activity of SA-Beta-Gal in cultured cells and tissue sections, which is a known characteristic of senescent cells. SA-Beta-Gal is only found in senescent cells, but not found in senescent, quiescent or immortal cells.
Features
•The procedure is simple; it takes about 30 minutes
•Fast and convenient
•Specific histochemical markers are only found in senescent cells, but not found in senescent, resting or immortal cells.
Applications
The aging detection kit is used for histochemical detection of specific aging markers at different pH values in cultured cells and tissue sections.
Size
250 Stainings
Sample
Cell culture (adherent and non adherent)
Species Reactivity
Mammalian
Components
Fixative Solution (1X)
X-Gal (150 mg, lyophilized)
Staining Solution (1X)
Staining Supplement (100X)
Shipping
Gel Pack
Storage
-20°C
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

Figure: HeLa cell senescence induced by hydrogen peroxide. 1X10 4 HeLa cells were seeded in (B) or untreated with 10 µM H 2 O 2 (A) in a 24-well plate for 6 days. Fix all cells in the fixative and stain overnight according to the kit protocol.

All products and services are for Research Use Only. Do Not use in humans.

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