Anti-Human C7 Monoclonal Antibody (clone WU 4-15) -0.5 mg (CAT#: CB-P327-AB) Datasheet

Product Type
Antibody
Description
The monoclonal antibody WU 4-15 recognizes C7, which is one of the components of the terminal complement complex (TCC), also known as the membrane attack complex (MAC). The proteolytic cleavage of C5 by C5 convertase produces C5b, which initiates the assembly of C5b-9 MAC. The complex is assembled from five precursor molecules in the serum, and finally with the polymerization of C9, the complex is inserted into the cell membrane to cause cell lysis. C7 occupies an important position in the TCC cascade. C7 undergoes a hydrophilic-amphiphilic transition after activation. It allows the developing MAC to directly bind to the target cell membrane. It has been demonstrated that C7 exhibits genetic polymorphism. Polymorphonuclear leukocytes represent the main source of C7. The fact that inflammatory cells have the potential to secrete C7 indicates that locally produced complement plays an important role in the inflammatory process. The monoclonal antibody WU 4-15 can recognize a specific allotype of C7: C7M. 62% of whites are homozygous for C7 M and 31% are heterozygous. Wu 4-15 antibody can quantify the contribution of locally synthesized C7 to the inflammatory process and identify allotypes.
Disease
Infectious diseases, Nephrology
Size
100 µg
Immunogen
Human C7
Species
Human
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
WU 4-15
Buffer
PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application
Flow cytometry, Immuno assays, Immuno fluorescence, Immuno precipitation, Western blot
Application Notes
For flow cytometry and western blotting, the diluent to be used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Target
C7
Alternative Names
C7; complement component 7; complement C7

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK