β(1-4)-galactosidase [For Glycan Release] (CAT#: CB-P265-K) Datasheet

Kit

The β-galactosidase of Streptococcus pneumoniae only releases the non-reducing terminal galactose linked to β(1-4) from complex carbohydrates and glycoproteins. So far, β(1-4) galactose is the most common linkage among N-linked oligosaccharides. For other galactosidase connections, it is recommended to use bovine testis β(1-3,4,6)-galactosidase (CB-P265-K catalog). The activity of this enzyme on four-antenna oligosaccharides is the same as that of disaccharides containing β(1-4) linked galactose. The fucose linked to the penultimate N-acetylglucosamine prevents the cleavage of galactose. Using 3 mU of enzyme, up to 100 µg of asialoferritin can be completely β(1-4)-delactosyl in less than 1 hour.

Source: Recombinant Streptococcus pneumoniae in E. coli

EC: 3.2.1.23

Alternate Names: β-D-galactoside galactohydrolase, Exo-(1-4)-β-D-galactanase, Lactase

Specific Activity:>6 U/mg
Activity:>3 U/mL

Molecular weight: ~250,000 daltons

Specificity: Non-reducing terminal β(1-4)-galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

Specific Activity Assay: One unit of β Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C pH 5 from p-nitrophenyl-β-D-galactopyranoside.

Non-reducing terminal β(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.