β(1-4)-galactosidase [For Glycan Release] (CAT#: CB-P265-K) Datasheet

Product Type
Kit
Description
The β-galactosidase of Streptococcus pneumoniae only releases the non-reducing terminal galactose linked to β(1-4) from complex carbohydrates and glycoproteins. So far, β(1-4) galactose is the most common linkage among N-linked oligosaccharides. For other galactosidase connections, it is recommended to use bovine testis β(1-3,4,6)-galactosidase (CB-P265-K catalog). The activity of this enzyme on four-antenna oligosaccharides is the same as that of disaccharides containing β(1-4) linked galactose. The fucose linked to the penultimate N-acetylglucosamine prevents the cleavage of galactose. Using 3 mU of enzyme, up to 100 µg of asialoferritin can be completely β(1-4)-delactosyl in less than 1 hour.
Features
Source: Recombinant Streptococcus pneumoniae in E. coli

EC: 3.2.1.23

Alternate Names: β-D-galactoside galactohydrolase, Exo-(1-4)-β-D-galactanase, Lactase

Specific Activity:>6 U/mg
Activity:>3 U/mL

Molecular weight: ~250,000 daltons

Specificity: Non-reducing terminal β(1-4)-galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

Specific Activity Assay: One unit of β Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C pH 5 from p-nitrophenyl-β-D-galactopyranoside.
Applications
Non-reducing terminal β(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.
Size
0.18 U / 60 µL; Sufficient for up to 60 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
β-(1-4) Galactosidase in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
5x Reaction Buffer 6.0 (250 mM sodium phosphate, pH 6.0).
Handling Advice
1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
2. Add water to 14 µL
3. Add 4 µL 5X Reaction Buffer
4. Add 2 µL β(1-4) Galactosidase
5. Incubate at 37°C for 1 hour.
Storage
Storage: Store enzyme at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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