CreaTag™ 2-AA (2-aminobenzoic acid) Glycan Labeling Kit, 2PB reductant [For Glycan Labeling] (CAT#: CB-P294-K) Datasheet

Product Type
Kit
Description
The kit contains reagents for coupling the dye to the free reducing end of the glycan through a reductive amination reaction.
Features
Number of Samples: 12 separate analytical samples per set of labeling reagents (24 samples in total per kit)
Amount of Sample: From 25 pmol up to 25 nmol glycans per sample.
Suitable Samples: Any purified glycans with free reducing termini can be labeled.
Structural Integrity: No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
Labeling Selectivity:Essentially stoichiometric labeling.
Applications
For labelling of free glycans with 2-aminobenzoic acid (2-AA). 2- picoline borane reductant. Kit sufficient for 24 samples.
Principle
Fluorescent labelling methods for glycoprofiling of therapeutic glycoproteins are well established and aid subsequent separation and quantitation using a range of techniques. These include high performance liquid chromatography (HPLC and UHPLC), capillary electrophoresis (CE), and mass spectrometry. Of the fluorescent labels available, 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) are most widely used for oligosaccharide profiling. Existing 2-AA and 2-AB labelling kits use sodium cyanoborohydride as a reducing agent during glycan labelling. This reagent is toxic so a fume cupboard should be used during handling. To conform with emerging Health and Safety regulations we are now replacing these with our new VP glycan kits that use picoline borane which is a significantly safer reductant. The VP kits have the same glycan labelling performance as the traditional kits but are safer, more convenient (fewer vials to mix) and the reagents are more stable.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
2-AA Dye (2-aminobenzoic acid) 7.5 mg
2PB reductant (2-picoline borane) 16.5 mg
30% acetic acid in DMSO 500 µl
Handling Advice
Ensure that any glass, plastic ware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate. • Once individual vials of reagents are opened, their contents should be used immediately and excess then discarded according to local safety rules.
Storage
Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The reagents are stable for at least two years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

Figure 1 Overlay of 48 replicates of 2AB-labelled human IgG glycans reduced with picoline borane reductant run on a HILIC UHPLC column. 2.5 µg of IgG glycans labelled for each replicate.

Figure 2: 2AB labelling of human IgG glycans run on a HILIC UHPLC column comparing 5 replicates of reduced with picoline borane reductant with 5 replicates reduced with sodium cyanoborohydride.

All products and services are for Research Use Only. Do Not use in humans.

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