Kit

The kit contains reagents for coupling the dye to the free reducing end of the glycan through a reductive amination reaction.

Number of Samples: 12 separate analytical samples per set of labeling reagents (24 samples in total per kit)
Amount of Sample: From 25 pmol up to 25 nmol glycans per sample.
Suitable Samples: Any purified glycans with free reducing termini can be labeled.
Structural Integrity: No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
Labeling Selectivity:Essentially stoichiometric labeling.

For labelling of free glycans with 2-aminobenzoic acid (2-AA). 2- picoline borane reductant. Kit sufficient for 24 samples.

Fluorescent labelling methods for glycoprofiling of therapeutic glycoproteins are well established and aid subsequent separation and quantitation using a range of techniques. These include high performance liquid chromatography (HPLC and UHPLC), capillary electrophoresis (CE), and mass spectrometry. Of the fluorescent labels available, 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) are most widely used for oligosaccharide profiling. Existing 2-AA and 2-AB labelling kits use sodium cyanoborohydride as a reducing agent during glycan labelling. This reagent is toxic so a fume cupboard should be used during handling. To conform with emerging Health and Safety regulations we are now replacing these with our new VP glycan kits that use picoline borane which is a significantly safer reductant. The VP kits have the same glycan labelling performance as the traditional kits but are safer, more convenient (fewer vials to mix) and the reagents are more stable.