Pyruvate Assay Kit (CAT#: CB-P145-K) Datasheet

Product Type
Kit
Description
The pyruvate assay kit (colorimetry/Fluorometric) provides a simple, direct and automated ready procedure for measuring in various biological samples (such as blood, cells, culture media, fermentation media, etc.) Pyruvate concentration.
In the pyruvate assay protocol, pyruvate is oxidized by pyruvate oxidase through an enzymatic reaction to produce color (λ = 570 nm) and Fluorometric (at Ex / Em = 535/587 nm). Since the color or Fluorometric intensity is directly proportional to the pyruvate content, the pyruvate concentration can be accurately measured.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 200 µM
Sensitivity
> 1 µM
Sample
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
Pyruvate Assay Buffer 1 x 25ml
Pyruvate Enzyme Mix (Lyophilized) 1 vial
Pyruvate Probe (in DMSO) 1 x 200µl
Pyruvate Standard (100 nmol/µl) 1 x 100µl
Requires
Microplate reader
Handling Advice
Pyruvate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Pyruvate measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample


Figure 2 Pyruvate measured colourimetrically in biological fluids showing quantity (nmol) per mL of tested sample


Figure 3 Pyruvate colorimetric standard curve.


Figure 4 Pyruvate fluorometric standard curve.

All products and services are for Research Use Only. Do Not use in humans.

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